Associate Scientist II, Research

Alnylam's Research department is seeking a highly motivated Associate Scientist II (ASII) to join our LC-MS (liquid chromatography - mass spectrometry) based omics team dedicated to discovering and validating novel RNAi therapeutics and developing clinically applicable biomarkers of target engagement and disease amelioration. The ASII will be responsible for implementing LC-MS based proteomics workflows using state-of-the-art instrumentation and data processing pipelines. The scope of the work will include, but is not limited to, mammalian cell culture, tissue processing, sample preparation and execution of targeted and global LC-MS proteomics analysis, trouble-shooting mass spectrometry equipment, quantitative peptide assay development, raw spectral data processing and multivariate data analysis. We are seeking an ambitious candidate with a strong mathematics and computational background in addition to wet lab experience for this position. The ASII will have strong interpersonal skills needed to successfully collaborate in a multi-disciplinary environment and provide support for several project teams.

This position is onsite and will be primarily located in Cambridge, MA.

Key Responsibilities

• Wet lab-based preparation of proteomes from cells, tissues, and biofluids for LC-MS analysis, including proteome extraction, protein digestion and peptide purification.
• Execution of and developmental support for LC-MS acquisition methods, including global data-independent acquisition (DIA) using an Orbitrap and/or TOF MS and targeted peptide analysis using a triple-quad MS.
• LC-MS troubleshooting and instrument maintenance including mobile phase preparation, instrument calibration, and system tuning and optimization.
• Processing of raw LC-MS spectral data for relative abundance of proteins in global proteomics experiments and absolute abundances of peptides in targeted experiments.
• Provide computational analysis of large -omics datasets for biologists, including differential expression analysis, multivariate statistical machine learning techniques and pathway/network enrichment analysis.
• Train and implement AI models for target prioritization based on wet lab findings, species translatability, and multi-omic datasets
• Maintain clear and organized laboratory notebook and communicate findings effectively to others in the Research organization.
• Possess a strong sense of teamwork and collaborate with a diverse and passionate group of scientists to bring innovate medicines to patients.

Qualifications

• MS or BS in Chemical Engineering, Biological Engineering, or related field, with 2+ years of wet lab experience, preferably in an industry setting.
• Basic understanding of analytical techniques (buffer preparation, quantification based on internal & external standards, chromatography and MS fundamentals) and familiarity with LC-MS-based omics technologies. Related hands-on experience is a plus.
• Programming and scientific computing experience in Python/R/Matlab with strong math and statistics skills is required.
• Experience with basic molecular biology techniques (cell culture, qPCR, western blot, etc.) is a plus.
• Experience working with ML/AI models is a plus.
• Excellent communication skills, detail oriented and organized, with the ability to thrive in a fast-paced team environment and handle multiple projects simultaneously.
  

About Alnylam:Alnylam Pharmaceuticals (Nasdaq: ALNY) has led the translation of RNA interference (RNAi) into a whole new class of innovative medicines with the potential to transform the lives of people afflicted with rare and more prevalent diseases. Based on Nobel Prize-winning science, RNAi therapeutics represent a powerful, clinically validated approach to treating diseases at their genetic source by "interfering" with mRNA that cause or contribute to disease. Since our founding in 2002, Alnylam has led the RNAi Revolution and continues to turn scientific possibility into reality.

Our culture:Our people-first culture is guided by our core values: fiercely innovative, open culture, purposeful urgency, passion for excellence, and commitment to people, and these values influence how we work and the business decisions we make. Thanks to feedback from our employees over the years, we've been fortunate to be named a top employer around the world. Alnylam is extremely proud to have been recognized as the #1 Large Employer by Boston Globe Top Places to Work in 2023 for the third consecutive year, one of Science Magazine's Top Biopharma Employers, one of America's Most Responsible Companies for 2024 by Newsweek, a Fast Company Best Workplace for Innovators, and a Great Place to Work in Canada, France, Italy, Spain, Switzerland, and UK - among others.

At Alnylam, we commit to an inclusive recruitment process and equal employment opportunity. We are dedicated to building an environment where employees can feel that they belong, can bring their authentic selves to work, and achieve to their full potential. By empowering employees to embrace their unique differences at work, our business grows stronger with advanced and original thinking, allowing us to bring groundbreaking medicines to patients. Qualified applicants will receive consideration for employment without regard to their race, color, religion, age, sex, sexual orientation, gender identity or expression, national origin, ethnicity, marital status, protected veteran status, disability, or any other characteristics prohibited by law.