Staff Research Associate - 135373

Under supervision, assist with experiments, make observations, and record experimental data related to research projects. Use molecular biological techniques to assist in the development of plasmid and viral based gene transfer vectors. Assist with animal studies including assisting with breeding transgenic mice, injections, and maintaining and screening transgenic mouse colony. Assist with in vivo fluorescent and bioluminescent imaging, necropsy, tissue trimming and histology. Prepare tissue cultures of primary lymphocytes and propagation of tumor cell lines. Extract RNA, reverse transcribe cDNA, perform RT-PCR, mini preps of plasmids and their analysis by gel. Perform basic cellular immunology tests to assess immunity such as FACS and CFSE proliferative assays, and automatic cells counting. Media and buffer preparation. Maintain lab records, and order supplies on an as needed basis.

• Theoretical knowledge of biochemistry, molecular biology, immunology and genetics, including relevant laboratory techniques, acquired through a Bachelor's degree in biology or related field; and/or equivalent combination of education and laboratory experience.

• Previous experience working in the field of Urology.

• Experience assisting with tissue culture including freezing, thawing, counting cells and splitting cultures.

• Ability to work with human tissues; tissue trimming, histology analysis, and knowledge in necropsy.

• Experience with data analysis using Excel and Prism software.

• Experience working with basic aseptic and tissue culture techniques.

• Ability to work with cell culture, media preparation, and DNA electrophoresis.

• Willingness to follow step-wise experimental protocols.

• Ability to use basic laboratory equipment including centrifuges, spectrophotometers, balances, pH meters, and electrophoresis equipment.

• Willingness to become familiar with proper handling of chemical and biohazard materials.

• Willingness to adhere to established UCSD guidelines for safety and environmental issues.

• Familiarity with computer software to analyze data, store results and compute raw data.

• Willingness to maintain confidentiality.

• Willingness to learn organizational skills as they relate to maintain lab equipment and supplies.

• Ability to perform basic PCR and ELISA techniques.

• Willingness to work with other staff members, students, faculty etc.

• Theoretical knowledge of normal and malignant hematopoietic stem and progenitor cell biology sufficient to aid in the design and interpretation of experiments on those topics.

• Theoretical knowledge of microbiology, cellular, molecular and prostate cancer biology.

• Knowledge of current prostate cancer disease models and understanding of clinical features of prostate cancer and treatments.

• Employment is subject to a criminal background check.

• Must be willing and able to work in BioSafety Level II environment.

• Must be willing and able to work with small animals and biohazardous materials.

• Must be willing and able to work with human cells.